Next-generation DNA sequencing (NGS) is an essential tool for understanding biodiversity.
The training units below provide an introduction to short read NGS (a.k.a. 2nd generation sequencing) on Illumina® platforms + the bioinformatics pipelines used to process data.
Prerequisites: Basic familiarity with the molecular laboratory (i.e. pipetting, DNA extraction, PCR) and command line utilities (i.e. Linux and MacOS ‘bash’ shells or Windows PuTTy command line).
Prerequisites: Basic familiarity with the molecular laboratory (i.e. pipetting, DNA extraction, PCR) and command line utilities (i.e. Linux and MacOS ‘bash’ shells or Windows PuTTy command line).
Unit 1: Introduction to short read sequencing and library preparation
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In this unit we will learn:
Unit 1 Lecture (personal use only): 
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NextSeq 500. Image courtesy of Illumina®
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Unit 2: Illumina libraries, de novo assembly and reference mapping
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In this unit we will learn:
Unit 2 Lecture (personal use only)
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New England Bio Labs reagents at the NHM
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Unit 3: Targeted sequence capture of ultraconserved elements (UCEs)
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In this unit we will learn:
Unit 3 Lecture (personal use only)
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Post-capture UCE libraries at the NHM
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Unit 4: Double digest restriction-site associated DNA sequencing (ddRADseq)
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In this unit we will learn:
Unit 4 Lecture (personal use only):
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DNA extractions for ddRADseq at the NHM
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Unit 5: Museum genomics using archival DNA + Illumina library preps (Coming Soon!)
Science Group | Room DC1 209B, Darwin Centre | Natural History Museum | London | SW7 5BD
